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KMID : 0043320170400030364
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Archives of Pharmacal Research
2017 Volume.40 No. 3 p.364 ~ p.372
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Determination of urazamide in pharmaceutical preparation with room temperature ionic liquid
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Seo Na-Rae
Lee Yoo-Rim
Park Hyun-Sun
Truong Quoc Ky
Lee Jae-Yong
Chung Hyun-Kyu
Choi Yu-Sung
Kim Byeong-Hee
Han Sang-Beom
Kim Kyeong-Ho
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Abstract
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A high performance liquid chromatographic method was developed and validated for the determination of urazamide in pharmaceutical preparation with novel green aqueous mobile phase modified with room temperature ionic liquids (RTILs). 1-Ethyl-3-methyl-imidazolium tetrafluoroborate ([EMIM][BF4]) was selected as a mobile phase additive to improve retention and avoid baseline disturbances at t0. Various mobile phase parameters such as cation moiety, chaotropic anion moiety, pH and concentration of RTILs were optimized to determine urazamide at the proper retention time. The assay was validated according to International Conference on Harmonization guidelines. The linearity of the calibration curve was good (r2 > 0.999). Intra-day precision varied between 0.50 and 1.23%. Relative standard deviations of inter-day precision ranged between 1.07 and 1.66%. Recoveries in tablets ranged between 99.7 and 101.2% and it was successfully applied to determine urazamide in pharmaceutical preparations.
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KEYWORD
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Very polar compound, Urazamide, Room temperature ionic liquids (RTILs), Reverse phase high performance liquid chromatography (RP-HPLC)
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